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Help for baseline fit and subtract
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Vivvi87
I am completely new to programming and I just started to learn Igor in order to write a procedures to process AFM force curves. Once the force curves are loaded (4 waves for each force curves, two y and two x), I want to fit a baseline on a subrange of the y waves and then subtract it. I created a wave where I list all the waves and then I go though it and I fit ones I want to fit (the first 2 every four, which are the y waves).
This is what I wrote:
Function Baseline_correction () string BaselineList = wavelist("*",";","") Make/T/N=(itemsinlist(BaselineList)) RawFC Wave_From_String (BaselineList, RawFC) variable i=0 variable j=0 for (i=0; i<numpnts(Root:Force_Curves:RawFC); i=i+4) CurveFit/Q/NTHR=0 line $(RawFC[i])[30,100] /X=$(RawFC[i+2]) /D CurveFit/Q/NTHR=0 line $(RawFC[i+1])[30,300] /X=$(RawFC[i+3]) /D endfor end function
Till here everything is fine, the curve fitting is done and a fit_wave wave is created for each of them. At this point though I am unable to subtract the fitting. I though about writing under the fitting command CurveFit this:
wave W_coef wave blfit blfit = W_coef[0] + W_coef[1]*x blfit -= $(RawFC[i])
But it doesn't work. I also tried this:
$RawFC[i+1]=$RawFC[i+1]-(W_coef[0]+W_coef[1]*$RawFC[i+3])
But again, It doesn't work. I get a message saying the $ cannot used this way (but even if I take it out there is another error).
If someone has any suggestion it would be really appreciated !!
Thanks in advance
May 7, 2018 at 07:49 am - Permalink
John Weeks
WaveMetrics, Inc.
support@wavemetrics.com
May 7, 2018 at 10:15 am - Permalink
thanks for your help. I tried to do as you suggested, but it doesn't do what I wanted to - maybe I didnt explain myself well, what I would like to do is:
or
The former works if I type it in the command line, but I don't know how to insert it into the procedure as to do it automatically to all my waves.
Thanks again.
May 7, 2018 at 11:56 pm - Permalink
May 8, 2018 at 08:12 am - Permalink