4Misc_Starth4Platformh@9VersionCheck uPICT_0tX' tlD' EMFt8}HCF, EMF+@``F EMF+0@?*@$??2@CC*@$??%@@ "@ @ $@ !@ *@$f,8f,8@T H PNG  IHDREsRGBgAMA a pHYsod IDATx^ׁ۸EwʡY,R 0M$Gdt2?~ @ E"BPA( @ E"BPA( @ E"BPA( @ E"BPA( @ Ed?~S#9_P#ѻW?g==Al(yuՕg=__fgG]quW!7BD5LU{dgA >c/vy#UgC$;#RϬA}g,}P_bjϪ=DA(E( LfzΞG6j,g䌐9s3Y2s~;]LϪ=D ٟ8kgٕsIlǪ=D#:=g%o7zi=5>j\ ,|:,ye{V==k+b$ hs?}r7Q{]Է3*śH(~ u~f_>Y `+Wځ9]|zz/,V*3+]9{ z/y JWbS&ځUFggV[s _~1 BqLhΧvZ55cCqJDVOe3g+Ҿv箙~%6%nط;cO~Wm9kۨúR[]KOw̞E`$/a^*y{ZUcOw<1̡蝍P{9ӻ/],z1C(&a:*3+2=O#EB"V+e; F3';iF<9蝉P˲U#gt(GOZo,c_M[jk`,Y~wO3Y%6)oV++<ǛzU}g Ƭ]fQ;[+]:oWÛ֮ٚV3C|B;oB,oGYxkfu-fؤq:zxl!7ߣ<=5Z;YP+Iuz}g{jyz5Z]dkU0a]azq<],}e lؤi:VWg9j}^L<~%6inUvYoZ\]x{PM[j5>+MʲB(R2TQ9gYiѪ,c/O9 ;VwxϳYi}3iUU5ߌ]ygs޼zDӪ*sүfc.^J^oVU ۱b˛oVUٙY萎=+͛Vѽ>[UaV(V,젢{c^o*T(ff7j>/o6ZڪJ3PCj5BlEUMG0 7{;Argo${gfvO&wGgXu "$w3:n$Q!QWx7| z<>ATy֨ g ""$Y#V>kg-"U"$Y#V> 7=PdD=ggV ZJ@QAQWX("H3FsG3p we87>؝@7=êspネh+j=/p+!we-+[}{k||A( @ E"BPA( @ E"BPA( @ E"BՉHIENDB`@$@@4CC$´ь0/Hr1H6[5s+Ȕc빅^JV}3vɦVVv+Zk@L} ;kV z +k2&-L#į畀)VEc}VHa3#ANXc`- vJ{X gz/+*-\%L2N陸 0*V# a ]JPFX1&XBނĕ4 " Hv0~SJ{l3n)6NJlJNch3u*>U[x_m=?M/?dl/sFaBF͖VJXC 9]yfpZYY kTya"-̜sbTaRL=,U\O B<% A/*{mAa`P!3|VX?9a( f µ,)oA:ap0KHZGyUgįՀT?fޭ@Iueu]&d)3.4xuIu3$bHJLe<%)7tSxQvR--)?QnĞLy p&Uhie{qHaZ ZڬB} W hioB~=Ƶ-yU&hiZ5&ha:(|N@0Aa`PAa`PAa`\' /sg00( 00( 00( 00( 00( 00( fBa`PAa`PAa`PAa`PAa`P7%:P3>*)RQ}V0N}(/a Ͻ4(Y!^¬xYaVOAYa.{haU(LS( aPXvR49ѤI"0gQ3Aa`Pv)ъJ_M*Aa`Pq00Z^V񫉂#E{ )( H#P$Aa*00( VW0UjBWQ^ESfk HaZhF}^X [X -ךdJ ӱBA%}r>B~;dSjjT+;xAىbi >eY@Me5@ o{UgoyJ@ k+ޢ> r 'Zcha;%=TRXó\J kxl&ZZ`+ycݑwNq.u%(\#a W ,!oAJaK*Q)!LߔlҞ) l7Bi J?Mȏќ Qҧ zK$y:(]Ӕ,׹2[|B)abF˘MSNp(쁓fɒVFyK43g"U1HfΈR*0:Oּ-UҬ>?= REͶ QIV00( >+ҟf^OAZap0KHPB%`$z- ty*pt3yj@L3V̲.rgӔT?<:Aꤺxg$%&2EޔTK:Qs(;YhޔTK(7bH`&'LCaP 00( W#}v:!8b簯Jÿj՞HߏGG.-G Ldn;YC܌u_7;ZC?VmͺlNb?h4b]출ˢ&SVUSS(2RX̩? Aa`PAa`PƧYۡ煡ia|V* Aa8BaPF0?),3PBa .v8|RX@MBa`PF0( O CFa`|V ASnxإP^CP"в(8, +%e w~]w"L+3Xu<%bSek;E I?HްegNx~ƙh:Q|jUf|zO$! +skrS2.,zSN~R,vkiٗvֳS/{;g [ӗ gz0A.[ gy%MXC -bs<*!%Dݳta\b٥z3K)a%~ߌRRX~όRVX~٥0һw:BXC.sqv8FXC 07C < |Ha -lv8Ve 97knivqW~),ڟ[]+LM7I0q!SdSmY DV=gYO kY!?].j$3̻֐b=T k3+OzNia yn &{}z>a y&5}+ dSn>-J)a;e{U70@(00( 00( :a0( 00( 00( 00( 00( 00( 0R 00( 00( ad bIENDB`@$@@4BD%{IJ!b !l  Q klP0@ l(l???s??><8 ???????????~>?????????????????????????????????????yg?????g?? w????????????????????????????????yg?????g????x???" FEMF+$@ *@$??*@$??&@@ 4@ Ldkl)??" FEMF+@  2PICT_22kx2lkx EMF28@ F, EMF+@``FEMF+0@?*@$??2@BD*@$??%@@ "@ @ $@ !@ *@$f,8f,8@D8PNG  IHDRl^sRGBgAMA a pHYsod IDATx^횋 D?툕Ә|ӧҝ! (@Aa`PAa`PAa`@ 믿܃Fɉb3Rǧ@MGN.{[Nzuٜƚa&ф`] "=ВnCN݂w?;xZZunja8boVd`PAa`PAa`PAa`PDP00( +L3J;LQMő تTK&L fy00(Ca`PGbdun´$+':/rTaORQ$tao(@M %#gxJ| /\K| /^,Z 0sha#dPBh V@XC*ŠkhiV)O&{2")ь* "H#xJIaYPhDAa Dq00--3̾>´ь0/Hr1H6[5s+Ȕc빅^JV}3vɦVVv+Zk@L} ;kV z +k2&-L#į畀)VEc}VHa3#ANXc`- vJ{X gz/+*-\%L2N陸 0*V# a ]JPFX1&XBނĕ4 " Hv0~SJ{l3n)6NJlJNch3u*>U[x_m=?M/?dl/sFaBF͖VJXC 9]yfpZYY kTya"-̜sbTaRL=,U\O B<% A/*{mAa`P!3|VX?9a( f µ,)oA:ap0KHZGyUgįՀT?fޭ@Iueu]&d)3.4xuIu3$bHJLe<%)7tSxQvR--)?QnĞLy p&Uhie{qHaZ ZڬB} W hioB~=Ƶ-yU&hiZ5&ha:(|N@0Aa`PAa`PAa`\' /sg00( 00( 00( 00( 00( 00( fBa`PAa`PAa`PAa`PAa`P7%:P3>*)RQ}V0N}(/a Ͻ4(Y!^¬xYaVOAYa.{haU(LS( aPXvR49ѤI"0gQ3Aa`Pv)ъJ_M*Aa`Pq00Z^V񫉂#E{ )( H#P$Aa*00( VW0UjBWQ^ESfk HaZhF}^X [X -ךdJ ӱBA%}r>B~;dSjjT+;xAىbi >eY@Me5@ o{UgoyJ@ k+ޢ> r 'Zcha;%=TRXó\J kxl&ZZ`+ycݑwNq.u%(\#a W ,!oAJaK*Q)!LߔlҞ) l7Bi J?Mȏќ Qҧ zK$y:(]Ӕ,׹2[|B)abF˘MSNp(쁓fɒVFyK43g"U1HfΈR*0:Oּ-UҬ>?= REͶ QIV00( >+ҟf^OAZap0KHPB%`$z- ty*pt3yj@L3V̲.rgӔT?<:Aꤺxg$%&2EޔTK:Qs(;YhޔTK(7bH`&'LCaP 00( W#}v:!8b簯Jÿj՞HߏGG.-G Ldn;YC܌u_7;ZC?VmͺlNb?h4b]출ˢ&SVUSS(2RX̩? Aa`PAa`PƧYۡ煡ia|V* Aa8BaPF0?),3PBa .v8|RX@MBa`PF0( O CFa`|V ASnxإP^CP"в(8, +%e w~]w"L+3Xu<%bSek;E I?HްegNx~ƙh:Q|jUf|zO$! +skrS2.,zSN~R,vkiٗvֳS/{;g [ӗ gz0A.[ gy%MXC -bs<*!%Dݳta\b٥z3K)a%~ߌRRX~όRVX~٥0һw:BXC.sqv8FXC 07C < |Ha -lv8Ve 97knivqW~),ڟ[]+LM7I0q!SdSmY DV=gYO kY!?].j$3̻֐b=T k3+OzNia yn &{}z>a y&5}+ dSn>-J)a;e{U70@(00( 00( :a0( 00( 00( 00( 00( 00( 0R 00( 00( ad bIENDB`@$@@4BD%{IJ!b !l  Q klP0@ l(l???s??><8 ???????????~>?????????????????????????????????????yg?????g?? w????????????????????????????????yg?????g????x???" FEMF+$@ *@$??*@$??&@@ 4@ Ldkl)??" FEMF+@  2PICT_32kx2lkx EMF28@ F, EMF+@``FEMF+0@?*@$??2@BD*@$??%@@ "@ @ $@ !@ *@$f,8f,8@D8PNG  IHDRl^sRGBgAMA a pHYsod IDATx^횋 D?툕Ә|ӧҝ! (@Aa`PAa`PAa`@ 믿܃Fɉb3Rǧ@MGN.{[Nzuٜƚa&ф`] "=ВnCN݂w?;xZZunja8boVd`PAa`PAa`PAa`PDP00( +L3J;LQMő تTK&L fy00(Ca`PGbdun´$+':/rTaORQ$tao(@M %#gxJ| /\K| /^,Z 0sha#dPBh V@XC*ŠkhiV)O&{2")ь* "H#xJIaYPhDAa Dq00--3̾>´ь0/Hr1H6[5s+Ȕc빅^JV}3vɦVVv+Zk@L} ;kV z +k2&-L#į畀)VEc}VHa3#ANXc`- vJ{X gz/+*-\%L2N陸 0*V# a ]JPFX1&XBނĕ4 " Hv0~SJ{l3n)6NJlJNch3u*>U[x_m=?M/?dl/sFaBF͖VJXC 9]yfpZYY kTya"-̜sbTaRL=,U\O B<% A/*{mAa`P!3|VX?9a( f µ,)oA:ap0KHZGyUgįՀT?fޭ@Iueu]&d)3.4xuIu3$bHJLe<%)7tSxQvR--)?QnĞLy p&Uhie{qHaZ ZڬB} W hioB~=Ƶ-yU&hiZ5&ha:(|N@0Aa`PAa`PAa`\' /sg00( 00( 00( 00( 00( 00( fBa`PAa`PAa`PAa`PAa`P7%:P3>*)RQ}V0N}(/a Ͻ4(Y!^¬xYaVOAYa.{haU(LS( aPXvR49ѤI"0gQ3Aa`Pv)ъJ_M*Aa`Pq00Z^V񫉂#E{ )( H#P$Aa*00( VW0UjBWQ^ESfk HaZhF}^X [X -ךdJ ӱBA%}r>B~;dSjjT+;xAىbi >eY@Me5@ o{UgoyJ@ k+ޢ> r 'Zcha;%=TRXó\J kxl&ZZ`+ycݑwNq.u%(\#a W ,!oAJaK*Q)!LߔlҞ) l7Bi J?Mȏќ Qҧ zK$y:(]Ӕ,׹2[|B)abF˘MSNp(쁓fɒVFyK43g"U1HfΈR*0:Oּ-UҬ>?= REͶ QIV00( >+ҟf^OAZap0KHPB%`$z- ty*pt3yj@L3V̲.rgӔT?<:Aꤺxg$%&2EޔTK:Qs(;YhޔTK(7bH`&'LCaP 00( W#}v:!8b簯Jÿj՞HߏGG.-G Ldn;YC܌u_7;ZC?VmͺlNb?h4b]출ˢ&SVUSS(2RX̩? Aa`PAa`PƧYۡ煡ia|V* Aa8BaPF0?),3PBa .v8|RX@MBa`PF0( O CFa`|V ASnxإP^CP"в(8, +%e w~]w"L+3Xu<%bSek;E I?HްegNx~ƙh:Q|jUf|zO$! +skrS2.,zSN~R,vkiٗvֳS/{;g [ӗ gz0A.[ gy%MXC -bs<*!%Dݳta\b٥z3K)a%~ߌRRX~όRVX~٥0һw:BXC.sqv8FXC 07C < |Ha -lv8Ve 97knivqW~),ڟ[]+LM7I0q!SdSmY DV=gYO kY!?].j$3̻֐b=T k3+OzNia yn &{}z>a y&5}+ dSn>-J)a;e{U70@(00( 00( :a0( 00( 00( 00( 00( 00( 0R 00( 00( ad bIENDB`@$@@4BD%{IJ!b !l  Q klP0@ l(l???s??><8 ???????????~>?????????????????????????????????????yg?????g?? w????????????????????????????????yg?????g????x???" FEMF+$@ *@$??*@$??&@@ 4@ Ldkl)??" FEMF+@  2PICT_42kx2lkx EMF28@ F, EMF+@``FEMF+0@?*@$??2@BD*@$??%@@ "@ @ $@ !@ *@$f,8f,8@D8PNG  IHDRl^sRGBgAMA a pHYsod IDATx^횋 D?툕Ә|ӧҝ! (@Aa`PAa`PAa`@ 믿܃Fɉb3Rǧ@MGN.{[Nzuٜƚa&ф`] "=ВnCN݂w?;xZZunja8boVd`PAa`PAa`PAa`PDP00( +L3J;LQMő تTK&L fy00(Ca`PGbdun´$+':/rTaORQ$tao(@M %#gxJ| /\K| /^,Z 0sha#dPBh V@XC*ŠkhiV)O&{2")ь* "H#xJIaYPhDAa Dq00--3̾>´ь0/Hr1H6[5s+Ȕc빅^JV}3vɦVVv+Zk@L} ;kV z +k2&-L#į畀)VEc}VHa3#ANXc`- vJ{X gz/+*-\%L2N陸 0*V# a ]JPFX1&XBނĕ4 " Hv0~SJ{l3n)6NJlJNch3u*>U[x_m=?M/?dl/sFaBF͖VJXC 9]yfpZYY kTya"-̜sbTaRL=,U\O B<% A/*{mAa`P!3|VX?9a( f µ,)oA:ap0KHZGyUgįՀT?fޭ@Iueu]&d)3.4xuIu3$bHJLe<%)7tSxQvR--)?QnĞLy p&Uhie{qHaZ ZڬB} W hioB~=Ƶ-yU&hiZ5&ha:(|N@0Aa`PAa`PAa`\' /sg00( 00( 00( 00( 00( 00( fBa`PAa`PAa`PAa`PAa`P7%:P3>*)RQ}V0N}(/a Ͻ4(Y!^¬xYaVOAYa.{haU(LS( aPXvR49ѤI"0gQ3Aa`Pv)ъJ_M*Aa`Pq00Z^V񫉂#E{ )( H#P$Aa*00( VW0UjBWQ^ESfk HaZhF}^X [X -ךdJ ӱBA%}r>B~;dSjjT+;xAىbi >eY@Me5@ o{UgoyJ@ k+ޢ> r 'Zcha;%=TRXó\J kxl&ZZ`+ycݑwNq.u%(\#a W ,!oAJaK*Q)!LߔlҞ) l7Bi J?Mȏќ Qҧ zK$y:(]Ӕ,׹2[|B)abF˘MSNp(쁓fɒVFyK43g"U1HfΈR*0:Oּ-UҬ>?= REͶ QIV00( >+ҟf^OAZap0KHPB%`$z- ty*pt3yj@L3V̲.rgӔT?<:Aꤺxg$%&2EޔTK:Qs(;YhޔTK(7bH`&'LCaP 00( W#}v:!8b簯Jÿj՞HߏGG.-G Ldn;YC܌u_7;ZC?VmͺlNb?h4b]출ˢ&SVUSS(2RX̩? Aa`PAa`PƧYۡ煡ia|V* Aa8BaPF0?),3PBa .v8|RX@MBa`PF0( O CFa`|V ASnxإP^CP"в(8, +%e w~]w"L+3Xu<%bSek;E I?HްegNx~ƙh:Q|jUf|zO$! +skrS2.,zSN~R,vkiٗvֳS/{;g [ӗ gz0A.[ gy%MXC -bs<*!%Dݳta\b٥z3K)a%~ߌRRX~όRVX~٥0һw:BXC.sqv8FXC 07C < |Ha -lv8Ve 97knivqW~),ڟ[]+LM7I0q!SdSmY DV=gYO kY!?].j$3̻֐b=T k3+OzNia yn &{}z>a y&5}+ dSn>-J)a;e{U70@(00( 00( :a0( 00( 00( 00( 00( 00( 0R 00( 00( ad bIENDB`@$@@4BD%{IJ!b !l  Q klP0@ l(l???s??><8 ???????????~>?????????????????????????????????????yg?????g?? w????????????????????????????????yg?????g????x???" FEMF+$@ *@$??*@$??&@@ 4@ Ldkl)??" FEMF+@  2PICT_52kx2lkx EMF28@ F, EMF+@``FEMF+0@?*@$??2@BD*@$??%@@ "@ @ $@ !@ *@$f,8f,8@D8PNG  IHDRl^sRGBgAMA a pHYsod IDATx^횋 D?툕Ә|ӧҝ! 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Aa`PAa`PƧYۡ煡ia|V* Aa8BaPF0?),3PBa .v8|RX@MBa`PF0( O CFa`|V ASnxإP^CP"в(8, +%e w~]w"L+3Xu<%bSek;E I?HްegNx~ƙh:Q|jUf|zO$! +skrS2.,zSN~R,vkiٗvֳS/{;g [ӗ gz0A.[ gy%MXC -bs<*!%Dݳta\b٥z3K)a%~ߌRRX~όRVX~٥0һw:BXC.sqv8FXC 07C < |Ha -lv8Ve 97knivqW~),ڟ[]+LM7I0q!SdSmY DV=gYO kY!?].j$3̻֐b=T k3+OzNia yn &{}z>a y&5}+ dSn>-J)a;e{U70@(00( 00( :a0( 00( 00( 00( 00( 00( 0R 00( 00( ad bIENDB`@$@@4BD%{IJ!b !l  Q klP0@ l(l???s??><8 ???????????~>?????????????????????????????????????yg?????g?? w????????????????????????????????yg?????g????x???" FEMF+$@ *@$??*@$??&@@ 4@ Ldkl)??" FEMF+@  2PICT_62kx2lkx EMF28@ F, EMF+@``FEMF+0@?*@$??2@BD*@$??%@@ "@ @ $@ !@ *@$f,8f,8@D8PNG  IHDRl^sRGBgAMA a pHYsod IDATx^횋 D?툕Ә|ӧҝ! 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FEMF+@  xHH%.7@gyHHdh xHH%.7@gyHHdh Layout0xHH%.7@gyHHdh x HH%.7@gyHHdh ^Graph*raWDashSettings#  ! 6Normal@ Lucida Console<HHHH$$4 4 4 4 4 4 homeydD:archived:Dropbox:Manuscripts:2019:01_HHQ+PQS_microITIES:Experimental_Data:Microcell_HHQ:D:archived:Dropbox:Manuscripts:2019:01_HHQ+PQS_microITIES:Experimental_Data:Microcell_HHQRecentWindowsErrors.ihfExperiments, Files and Folders.ihfGraph0:i_pH4_blk vs E_pH4_blk;...Graph0_1:i_pH3p5_blk vs E_pH3p5_blk;...Graph0_2:i_pH3_blk vs E_pH3_blk;...Graph0_3:i_pH2p5_blk vs E_pH2p5_blk;...Graph1:i_pH3_100uM_HHQ vs E_pH3_100uM_HHQ_2;...Graph2:i_pH2p5_010umHHQ vs E_pH2p5_010umHHQ;...Graph3:i_pH2p5_025umHHQ vs E_pH2p5_025umHHQGraph4:i_pH2p5_100umHHQ vs E_pH2p5_100umHHQGraph5:i_pH4_100uM_HHQ vs E_pH4_100uM_HHQ;...Graph6:i_pH3_100uM_HHQ vs E_pH3_100uM_HHQ_2Graph7:i_pH3p5_100uM_HHQ vs E_pH3p5_100uM_HHQGraph8:i_pH4_100uM_HHQ vs E_pH4_100uM_HHQGraph13:i_pH2p5_050umHHQ vs E_pH2p5_050umHHQGraph15:i_pH3p5_050uM_HHQ vs E_pH3p5_050uM_HHQ;... 4Misc_EndhTXOPState_Start hPeakFunctions2-644XOPState_EndhAȢ?V_FlagV_sigamH?V_sigb(?V_chisqX?V_q?V_Rabn@tp?V_PreC ?V_r2uG?V_numNaNsV_numINFsV_npnts@V_nterms@V_nheldV_startRowV_endRow@V_startColV_endColV_startLayerV_endLayerV_startChunkV_endChunkS_waveNamesEhalf_HHQ_050uM;S_pathamesS_fileName ClipboardS_nameame UserFrontI>•Edit •Rename wave0,E_pH4_blk; Rename wave1,i_pH4_blk •Display i_pH4_blk vs E_pH4_blk •ModifyGraph manTick(left)={0,50,-12,0},manMinor(left)={1,50} •Rename wave0,E_pH4_010uM_HHQ; Rename wave1,i_pH4_010uM_HHQ •AppendToGraph i_pH4_010uM_HHQ vs E_pH4_010uM_HHQ •DeletePoints 381,235, E_pH4_010uM_HHQ,i_pH4_010uM_HHQ •DeletePoints 391,114, E_pH4_blk,i_pH4_blk •Rename wave0,E_pH4_025uM_HHQ; Rename wave1,i_pH4_025uM_HHQ •AppendToGraph i_pH4_025uM_HHQ vs E_pH4_025uM_HHQ •DeletePoints 391,60, E_pH4_025uM_HHQ,i_pH4_025uM_HHQ •DeletePoints 0,391, E_pH4_025uM_HHQ,i_pH4_025uM_HHQ •ModifyGraph rgb(i_pH4_025uM_HHQ)=(0,0,65535) •ShowInfo •DeletePoints 392,59, E_pH4_025uM_HHQ,i_pH4_025uM_HHQ •Rename wave0,E_pH4_050uM_HHQ; Rename wave1,i_pH4_050uM_HHQ •Rename wave0,E_pH4_100uM_HHQ; Rename wave1,i_pH4_100uM_HHQ •AppendToGraph i_pH4_050uM_HHQ vs E_pH4_050uM_HHQ •AppendToGraph i_pH4_100uM_HHQ vs E_pH4_100uM_HHQ •SetAxis/A •ModifyGraph manTick(left)={0,200,-12,0},manMinor(left)={0,50} •Rename wave0,E_pH4_500uM_HHQ; Rename wave1,i_pH4_500uM_HHQ •AppendToGraph i_pH4_500uM_HHQ vs E_pH4_500uM_HHQ •Rename wave0,E_pH4_250uM_HHQ; Rename wave1,i_pH4_250uM_HHQ •AppendToGraph i_pH4_250uM_HHQ vs E_pH4_250uM_HHQ •DeletePoints 417,346, E_pH4_500uM_HHQ,i_pH4_500uM_HHQ •Rename wave0,conc_HHQ; Rename wave1,j_uAcm2 •DisplayHelpTopic "Merging Experiments" Starting merge of experiment "2019_1029_pH3p5_tjs.pxp" Skipped loading the history of the merged experiment. Ending merge of experiment "2019_1029_pH3p5_tjs.pxp" Starting merge of experiment "2019_0930_pH_3p0_tjs.pxp" Skipped loading the history of the merged experiment. Ending merge of experiment "2019_0930_pH_3p0_tjs.pxp" Starting merge of experiment "2019_0930_pH_2p5_tjs.pxp" Skipped loading the history of the merged experiment. Ending merge of experiment "2019_0930_pH_2p5_tjs.pxp" •Display i_pH4_100uM_HHQ vs E_pH4_100uM_HHQ; AppendToGraph :'2019_0930_pH_2p5_tjs':i_pH2p5_100umHHQ vs :'2019_0930_pH_2p5_tjs':E_pH2p5_100umHHQ; AppendToGraph :'2019_0930_pH_3p0_tjs':i_pH3_100uM_HHQ vs :'2019_0930_pH_3p0_tjs':E_pH3_100uM_HHQ_2; AppendToGraph :'2019_1029_pH3p5_tjs':i_pH3p5_100uM_HHQ vs :'2019_1029_pH3p5_tjs':E_pH3p5_100uM_HHQ •ShowInfo •Edit •DeletePoints 4,1, wave0 •Rename wave0,pH •duplicate pH, conc_H •AppendToTable conc_H •conc_H=10^-(pH) •ModifyGraph rgb(i_pH2p5_100umHHQ)=(1,4,52428),rgb(i_pH3_100uM_HHQ)=(1,52428,26586),rgb(i_pH3p5_100uM_HHQ)=(0,0,0) •SetAxis/A •SetAxis/A •SetAxis/A •Print -4.0732e-10/2 -2.0366e-10 •SetAxis/A •ShowInfo •Display :'2019_0930_pH_3p0_tjs':i_pH3_100uM_HHQ vs :'2019_0930_pH_3p0_tjs':E_pH3_100uM_HHQ_2 •ShowInfo •print 3.7952e-10/2 1.8976e-10 •print (0.02719+0.03229)/2 0.02974 •Display :'2019_1029_pH3p5_tjs':i_pH3p5_100uM_HHQ vs :'2019_1029_pH3p5_tjs':E_pH3p5_100uM_HHQ •ShowInfo •print 3.667e-10/2 1.8335e-10 •Display i_pH4_100uM_HHQ vs E_pH4_100uM_HHQ •ShowInfo •print 3.5733e-10/2 1.78665e-10 •Rename wave0,Ehalf_100uM •duplicate conc_H, ln_cH •ln_cH=ln(conc_H) •AppendToTable ln_cH •jane01(Ehalf_100uM) •AppendToTable temp •Rename temp,rey_100uM •jane01(Ehalf_100uM) •AppendToTable rey_050uM •Display rey_100uM vs ln_cH •ModifyGraph manTick(left)={0,0.5,0,1},manMinor(left)={0,50},manTick(bottom)={0,0.5,0,1},manMinor(bottom)={0,50} •ModifyGraph mode=3,marker=8,msize=2.5,rgb=(0,0,0) •Display i_pH4_050uM_HHQ vs E_pH4_050uM_HHQ •ShowInfo •print 1.8076e-10/2 9.038e-11 •Rename wave0,Ehalf_050uM •Display :'2019_1029_pH3p5_tjs':i_pH3p5_050uM_HHQ vs :'2019_1029_pH3p5_tjs':E_pH3p5_050uM_HHQ •ShowInfo •print 2.0319e-10/2 1.01595e-10 •Display :'2019_0930_pH_3p0_tjs':i_pH3_050uM_HHQ vs :'2019_0930_pH_3p0_tjs':E_pH3_050uM_HHQ •ShowInfo •print 1.9061e-10/2 9.5305e-11 •Display :'2019_0930_pH_2p5_tjs':i_pH2p5_050umHHQ vs :'2019_0930_pH_2p5_tjs':E_pH2p5_050umHHQ •ShowInfo •print 2.0032e-10/2 1.0016e-10 •jane01(Ehalf_050uM) •AppendToTable rey_050uM •Display rey_050uM vs ln_cH •ModifyGraph manTick(left)={0,0.5,0,1},manMinor(left)={0,50},manTick(bottom)={0,0.5,0,1},manMinor(bottom)={0,50} •ModifyGraph mode=3,marker=8,msize=2.5,rgb=(0,0,0) •CurveFit line rey_050uM /X=ln_cH /D fit_rey_050uM= W_coef[0]+W_coef[1]*x W_coef={28.958,1.0632} V_chisq= 0.0352798;V_npnts= 4;V_numNaNs= 0;V_numINFs= 0; V_startRow= 0;V_endRow= 3;V_q= 1;V_Rab= 0.985527;V_Pr= 0.997654; V_r2= 0.995313; W_sigma={0.392,0.0516} Coefficient values ± one standard deviation a =28.958 ± 0.392 b =1.0632 ± 0.0516 •Display :'2019_1029_pH3p5_tjs':i_pH3p5_050uM_HHQ vs :'2019_1029_pH3p5_tjs':E_pH3p5_050uM_HHQ; AppendToGraph :'2019_0930_pH_3p0_tjs':i_pH3_050uM_HHQ vs :'2019_0930_pH_3p0_tjs':E_pH3_050uM_HHQ; AppendToGraph :'2019_0930_pH_2p5_tjs':i_pH2p5_050umHHQ vs :'2019_0930_pH_2p5_tjs':E_pH2p5_050umHHQ; AppendToGraph i_pH4_050uM_HHQ vs E_pH4_050uM_HHQ •ModifyGraph rgb(i_pH3p5_050uM_HHQ)=(4369,4369,4369),mode(i_pH3_050uM_HHQ)=3,marker(i_pH3_050uM_HHQ)=19,rgb(i_pH3_050uM_HHQ)=(3,52428,1),rgb(i_pH2p5_050umHHQ)=(0,0,65535) •ModifyGraph msize(i_pH3_050uM_HHQ)=2 •ModifyGraph mode=0,lstyle(i_pH3_050uM_HHQ)=8 •ModifyGraph lstyle(i_pH3_050uM_HHQ)=3 •ModifyGraph lstyle(i_pH3_050uM_HHQ)=2,lsize(i_pH3_050uM_HHQ)=2 •AppendToGraph i_pH4_blk vs E_pH4_blk •ModifyGraph lstyle(i_pH4_blk)=7,rgb(i_pH4_blk)=(0,0,0) •ModifyGraph manTick(left)={0,0.1,-9,1},manMinor(left)={1,50} •ShowTools/A arrow •Label left "\\F'Times New Roman'\\f02I\\f00 \\u#2/nA" •Label bottom "\\u#2" •TextBox/C/N=text0/F=0/M/A=MC "2.5 pH" •TextBox/C/N=text0/B=1 •TextBox/C/N=text0_1/F=0/B=1/M/A=MC/X=-33.09/Y=-5.24 "4.0 pH" •SetAxis bottom -0.13156015,0.45 •SetAxis bottom -0.13156015,0.5 •SetAxis left -9.2825767e-11,5.5e-10 •SetAxis left -9.2825767e-11,6e-10 •SetAxis bottom -0.13156015,0.55 •TextBox/C/N=text0 "a" •TextBox/C/N=text0_2/F=0/B=1/M/A=MC/X=19.85/Y=42.86 "b" •TextBox/C/N=text0_3/F=0/B=1/M/A=MC/X=37.13/Y=44.76 "c" •TextBox/C/N=text0_1 "d" •TextBox/C/N=text1/F=0/B=1/M/A=MC "blank" •SavePICT/E=-5/TRAN=1/B=288 as "CVs_50uM_HHQ.png" •ModifyGraph lstyle(i_pH4_blk)=3 •SavePICT/E=-5/TRAN=1/B=288 •SavePICT/E=-5/TRAN=1/B=288 •Display i_pH4_025uM_HHQ vs E_pH4_025uM_HHQ; AppendToGraph :'2019_1029_pH3p5_tjs':i_pH3p5_025uM_HHQ vs :'2019_1029_pH3p5_tjs':E_pH3p5_025uM_HHQ; AppendToGraph :'2019_0930_pH_3p0_tjs':i_pH3_025uM_HHQ vs :'2019_0930_pH_3p0_tjs':E_pH3_025uM_HHQ; AppendToGraph :'2019_0930_pH_2p5_tjs':i_pH2p5_025umHHQ vs :'2019_0930_pH_2p5_tjs':E_pH2p5_025umHHQ •ModifyGraph rgb(i_pH3p5_025uM_HHQ)=(4369,4369,4369),lstyle(i_pH3_025uM_HHQ)=2,lsize(i_pH3_025uM_HHQ)=2,rgb(i_pH3_025uM_HHQ)=(3,52428,1),rgb(i_pH2p5_025umHHQ)=(0,0,65535) •ShowInfo •SetAxis/A •SetAxis/A •print 7.666e-11/2 3.833e-11 •SetAxis/A •print 7.6233e-11/2 3.81165e-11 •Rename wave0,Ehalf_025uM •SetAxis/A •SetAxis/A •print 1.0175e-10/2 5.0875e-11 •SetAxis/A •SetAxis/A •SetAxis/A •print 9.674e-11/2 4.837e-11 •SetAxis/A •SetAxis/A •SetAxis/A •print 1.0269e-10/2 5.1345e-11 •jane01(Ehalf_025uM) •AppendToTable rey_025uM •Display rey_025uM vs ln_cH •ModifyGraph manTick(left)={0,1,0,0},manMinor(left)={0,50},manTick(bottom)={0,0.5,0,1},manMinor(bottom)={1,50} •ModifyGraph mode=3,marker=19,msize=2,rgb=(0,0,0) •CurveFit line rey_025uM /X=ln_cH /D fit_rey_025uM= W_coef[0]+W_coef[1]*x W_coef={29.918,1.1568} V_chisq= 1.01214;V_npnts= 4;V_numNaNs= 0;V_numINFs= 0; V_startRow= 0;V_endRow= 3;V_q= 1;V_Rab= 0.985527;V_Pr= 0.947397; V_r2= 0.89756; W_sigma={2.1,0.276} Coefficient values ± one standard deviation a =29.918 ± 2.1 b =1.1568 ± 0.276 •Display rey_100uM vs ln_cH •ModifyGraph mode=3,marker=19,msize=2,rgb=(0,0,0) •ModifyGraph manTick(left)={0,0.5,0,1},manMinor(left)={0,50},manTick(bottom)={0,0.5,0,1},manMinor(bottom)={0,50} •CurveFit line rey_100uM /X=ln_cH /D fit_rey_100uM= W_coef[0]+W_coef[1]*x W_coef={28.076,1.0322} V_chisq= 0.749727;V_npnts= 4;V_numNaNs= 0;V_numINFs= 0; V_startRow= 0;V_endRow= 3;V_q= 1;V_Rab= 0.985527;V_Pr= 0.950795; V_r2= 0.904011; W_sigma={1.81,0.238} Coefficient values ± one standard deviation a =28.076 ± 1.81 b =1.0322 ± 0.238 •ShowInfo •ShowInfo •SetAxis/A •print 2.9999e-11/2+7.9041e-12 2.29036e-11 •print 4.3793e-11/2+1.3519e-11 3.54155e-11 •Rename wave0,Ehalf_pH2p5 •SetAxis/A •print 9.3018e-11/2+2.2949e-11 6.9458e-11 •SetAxis/A •SetAxis/A •print 2.0721e-10/2+1.5503e-11 1.19108e-10 •Rename wave0,pH2p5_ligand_conc •print 4.0646e-10/2+1.5839e-11 2.19069e-10 •SetAxis/A •DeletePoints 5,1, pH2p5_ligand_conc •duplicate pH2p5_ligand_conc, ln_pH2p5_ligand_conc •ln_pH2p5_ligand_conc=ln(pH2p5_ligand_conc) •AppendToTable ln_pH2p5_ligand_conc •jane01(Ehalf_pH2p5) •AppendToTable rey_pH2p5 •Display rey_pH2p5 vs ln_pH2p5_ligand_conc •Display i_pH4_025uM_HHQ vs E_pH4_025uM_HHQ; AppendToGraph :'2019_1029_pH3p5_tjs':i_pH3p5_025uM_HHQ vs :'2019_1029_pH3p5_tjs':E_pH3p5_025uM_HHQ; AppendToGraph :'2019_0930_pH_3p0_tjs':i_pH3_025uM_HHQ vs :'2019_0930_pH_3p0_tjs':E_pH3_025uM_HHQ; AppendToGraph :'2019_0930_pH_2p5_tjs':i_pH2p5_025umHHQ vs :'2019_0930_pH_2p5_tjs':E_pH2p5_025umHHQ •ModifyGraph rgb(i_pH4_025uM_HHQ)=(0,0,65535),rgb(i_pH3p5_025uM_HHQ)=(26205,52428,1),rgb(i_pH3_025uM_HHQ)=(0,0,0) •ShowInfo •Edit pH,ln_cH •print 7.5531e-11/2+1.297e-11 5.07355e-11 •Rename wave0,Ehalf_025uM_ver2 •print 1.0001e-10/2+1.2329e-11 6.2334e-11 •SetAxis/A •print 9.3293e-11/2+1.4648e-11 6.12945e-11 •print 9.8175e-11/2+1.8799e-11 6.78865e-11 •Display i_pH4_050uM_HHQ vs E_pH4_050uM_HHQ; AppendToGraph :'2019_1029_pH3p5_tjs':i_pH3p5_050uM_HHQ vs :'2019_1029_pH3p5_tjs':E_pH3p5_050uM_HHQ; AppendToGraph :'2019_0930_pH_3p0_tjs':i_pH3_050uM_HHQ vs :'2019_0930_pH_3p0_tjs':E_pH3_050uM_HHQ; AppendToGraph :'2019_0930_pH_2p5_tjs':i_pH2p5_050umHHQ vs :'2019_0930_pH_2p5_tjs':i_pH2p5_050umHHQ •RemoveFromGraph i_pH2p5_050umHHQ •AppendToGraph i_pH4_050uM_HHQ[0,332] vs :'2019_0930_pH_2p5_tjs':E_pH2p5_050umHHQ •ModifyGraph rgb(i_pH4_050uM_HHQ)=(0,0,65535),rgb(i_pH3p5_050uM_HHQ)=(26205,52428,1),rgb(i_pH3_050uM_HHQ)=(0,0,0) •ShowInfo •SetAxis/A •print 1.8146e-10/2+1.4191e-11 1.04921e-10 •Rename wave0,Ehalf_050uM_ver2 •SetAxis/A •print 2.0282e-10/2+1.2695e-11 1.14105e-10 •print 1.9095e-10/2+1.947e-11 1.14945e-10 •SetAxis/A •print 1.8185e-10/2+1.4191e-11 1.05116e-10 •Display i_pH4_100uM_HHQ vs E_pH4_100uM_HHQ; AppendToGraph :'2019_1029_pH3p5_tjs':i_pH3p5_100uM_HHQ vs :'2019_1029_pH3p5_tjs':E_pH3p5_100uM_HHQ; AppendToGraph :'2019_0930_pH_3p0_tjs':i_pH3_100uM_HHQ vs :'2019_0930_pH_3p0_tjs':E_pH3_100uM_HHQ_2; AppendToGraph :'2019_0930_pH_2p5_tjs':i_pH2p5_100umHHQ vs :'2019_0930_pH_2p5_tjs':E_pH2p5_100umHHQ •ShowInfo •print 3.3701e-10/2+2.0142e-11 1.88647e-10 •Rename wave0,Ehalf_100uM_ver2 •ModifyGraph rgb(i_pH4_100uM_HHQ)=(0,0,65535),rgb(i_pH3p5_100uM_HHQ)=(26205,52428,1),rgb(i_pH3_100uM_HHQ)=(0,0,0) •SetAxis/A •print 3.6649e-10/2+1.4984e-11 1.98229e-10 •print 3.8101e-10/2+1.535e-11 2.05855e-10 •print 4.0582e-10/2+1.4893e-11 2.17803e-10 •jane01(Ehalf_025uM_ver2) •AppendToTable rey_025uM_02 •jane01(Ehalf_050uM_ver2) •AppendToTable rey_050uM_02 •jane01(Ehalf_100uM_ver2) •AppendToTable rey_100uM_02 •Display rey_025uM_02,rey_050uM_02,rey_100uM_02 vs ln_cH •ModifyGraph mode=3,rgb=(0,0,0),marker(rey_025uM_02)=19,marker(rey_050uM_02)=16,marker(rey_100uM_02)=17 •CurveFit line rey_025uM_02 /X=ln_cH /D fit_rey_025uM_02= W_coef[0]+W_coef[1]*x W_coef={29.567,1.2081} V_chisq= 0.549603;V_npnts= 4;V_numNaNs= 0;V_numINFs= 0; V_startRow= 0;V_endRow= 3;V_q= 1;V_Rab= 0.985527;V_Pr= 0.972746; V_r2= 0.946234; W_sigma={1.55,0.204} Coefficient values ± one standard deviation a =29.567 ± 1.55 b =1.2081 ± 0.204 •CurveFit line rey_050uM_02 /X=ln_cH /D fit_rey_050uM_02= W_coef[0]+W_coef[1]*x W_coef={29.585,1.1992} V_chisq= 0.242731;V_npnts= 4;V_numNaNs= 0;V_numINFs= 0; V_startRow= 0;V_endRow= 3;V_q= 1;V_Rab= 0.985527;V_Pr= 0.987505; V_r2= 0.975166; W_sigma={1.03,0.135} Coefficient values ± one standard deviation a =29.585 ± 1.03 b =1.1992 ± 0.135 •CurveFit line rey_100uM_02 /X=ln_cH /D fit_rey_100uM_02= W_coef[0]+W_coef[1]*x W_coef={28.188,1.0896} V_chisq= 0.674614;V_npnts= 4;V_numNaNs= 0;V_numINFs= 0; V_startRow= 0;V_endRow= 3;V_q= 1;V_Rab= 0.985527;V_Pr= 0.959703; V_r2= 0.92103; W_sigma={1.71,0.226} Coefficient values ± one standard deviation a =28.188 ± 1.71 b =1.0896 ± 0.226 •Display/L=rey25uM rey_025uM_02 vs ln_cH; AppendToGraph/L=rey50uM rey_050uM_02 vs ln_cH; AppendToGraph rey_100uM_02 vs ln_cH •ModifyGraph axisEnab(rey25uM)={0.66,1},axisEnab(rey50uM)={0.33,0.65},axisEnab(left)={0,0.32} •ModifyGraph tick(rey25uM)=2,tick(rey50uM)=2,mirror(rey25uM)=1,freePos(rey25uM)={0,kwFraction},freePos(rey50uM)={0,kwFraction} •ModifyGraph mirror(rey50uM)=1,mirror(left)=1 •ModifyGraph tick(bottom)=2,mirror=1 •ModifyGraph manTick(left)={0,1,0,0},manMinor(left)={1,50} •ModifyGraph tick=2 •Label bottom "ln[c\\BH\\S+\\M]" •ModifyGraph gFont="Times New Roman",gfSize=15 •ShowTools/A arrow •ModifyGraph mode=3,rgb=(0,0,0),marker(rey_025uM_02)=19,marker(rey_050uM_02)=16,marker(rey_100uM_02)=17,msize(rey_100uM_02)=2.5 •ModifyGraph msize(rey_100uM_02)=3 •ModifyGraph msize(rey_100uM_02)=4 •SetAxis/A/N=2 bottom •SetAxis/A/N=2 rey25uM;DelayUpdate •SetAxis/A/N=2 rey50uM;DelayUpdate •SetAxis/A/N=2 left;DelayUpdate •ModifyGraph axisEnab(rey25uM)={0.67,1},axisEnab(rey50uM)={0.34,0.65} •ModifyGraph axisEnab(rey25uM)={0.68,1},axisEnab(rey50uM)={0.35,0.64},axisEnab(left)={0,0.31} •ModifyGraph fSize(bottom)=14,fSize(left)=14 •ModifyGraph fSize=14 •ModifyGraph manTick(rey25uM)={0,2,0,0},manMinor(rey25uM)={1,50},manTick(rey50uM)={0,2,0,0},manMinor(rey50uM)={1,50},axisEnab(rey25uM)={0.69,1},axisEnab(rey50uM)={0.35,0.63},axisEnab(left)={0,0.3} •CurveFit line rey_100uM_02 /X=ln_cH /D fit_rey_100uM_02= W_coef[0]+W_coef[1]*x W_coef={28.188,1.0896} V_chisq= 0.674614;V_npnts= 4;V_numNaNs= 0;V_numINFs= 0; V_startRow= 0;V_endRow= 3;V_q= 1;V_Rab= 0.985527;V_Pr= 0.959703; V_r2= 0.92103; W_sigma={1.71,0.226} Coefficient values ± one standard deviation a =28.188 ± 1.71 b =1.0896 ± 0.226 •CurveFit line rey_050uM_02 /X=ln_cH /D fit_rey_050uM_02= W_coef[0]+W_coef[1]*x W_coef={29.585,1.1992} V_chisq= 0.242731;V_npnts= 4;V_numNaNs= 0;V_numINFs= 0; V_startRow= 0;V_endRow= 3;V_q= 1;V_Rab= 0.985527;V_Pr= 0.987505; V_r2= 0.975166; W_sigma={1.03,0.135} Coefficient values ± one standard deviation a =29.585 ± 1.03 b =1.1992 ± 0.135 •CurveFit line rey_025uM_02 /X=ln_cH /D fit_rey_025uM_02= W_coef[0]+W_coef[1]*x W_coef={29.567,1.2081} V_chisq= 0.549603;V_npnts= 4;V_numNaNs= 0;V_numINFs= 0; V_startRow= 0;V_endRow= 3;V_q= 1;V_Rab= 0.985527;V_Pr= 0.972746; V_r2= 0.946234; W_sigma={1.55,0.204} Coefficient values ± one standard deviation a =29.567 ± 1.55 b =1.2081 ± 0.204 •TextBox/C/N=text0/F=0/A=MC "\\Z18\\f01A" •TextBox/C/N=text0_1/F=0/A=MC/X=-40.07/Y=43.51 "\\Z18\\f01B" •TextBox/C/N=text0_2/F=0/A=MC/X=-40.75/Y=9.09 "\\Z18\\f01C" •ModifyGraph manTick(left)={0,2,0,0},manMinor(left)={1,50} •TextBox/C/N=text0/X=-40.00/Y=43.51 •TextBox/C/N=text0_1/X=-40.00/Y=9.09 •TextBox/C/N=text0_2/X=-40.00/Y=-23.70 •SavePICT/E=-5/B=72 as "HHQ_linear_fit" •SavePICT/E=-7/B=72 •SavePICT/E=-5/B=72 •SavePICT/E=-5/B=72 Graph24 inserted into Graph24 as Graph24#Graph24 •SetDrawEnv fillpat= 0 •SavePICT/E=-5/B=72 •SetDrawEnv linefgc= (65535,65535,65535) •Layout Graph24 •ModifyLayout frame=0 •ModifyLayout trans(PICT_4)=1 •SavePICT/E=-5/B=72 •SavePICT/E=-5/B=72 •ModifyGraph manTick(bottom)={0,1,0,0},manMinor(bottom)={1,50} •SavePICT/E=-5/B=72 ! ԩx 0ٻهE_pH4_blk????bڏa0r.ŵ:dw"?L F%u?e6? ?2ı.n?s`?ꭁ?x@e?%?al!A ?Z![?Ɏ@?oB!?:T?&?h㈵?VCK?V-?V-?yC?wE?+0du?D9?R%?U 7?P}:3?i?£#?nض(?`|?P1?dF ?QfL2r?eN?Ĭ?~8gDi?$ ?:u<?-9(a?Q,?F?&S?҇.?&S?F? 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A&2@nU%4@ti^k5@";5@4`0ٻTٱUEhalf_050uM????JR?͒5l?S"^F?&:@`0ٻUUrey_050uM????cx=3@!dV4@,IX7|5@ 6@P0ٻ@L@LW_coef????"=@iXT?KP50ٻVVfit_rey_050uM @???Uk"X O*3@҆Pg6@fit_rey_050uM= W_coef[0]+W_coef[1]*x W_coef={28.958,1.0632} V_chisq= 0.0352798;V_npnts= 4;V_numNaNs= 0;V_numINFs= 0; V_startRow= 0;V_endRow= 3;V_q= 1;V_Rab= 0.985527;V_Pr= 0.997654; V_r2= 0.995313; W_sigma={0.392,0.0516} Coefficient values ± one standard deviation a =28.958 ± 0.392 b =1.0632 ± 0.0516 ~P0ٻ@L@LW_sigma????mH?(?`1ٻ݈Ehalf_HHQ_050uM?????X9v?{Gz?;On?a`<1ٻwEhalf_025uM????)'$?~:p?֨ht?C6`/1ٻ٤rey_025uM????~eW3@jC4@˿H5@e7@͌P,@1ٻfit_rey_025uM @???Uk"T :dC3@;v_#3B7@fit_rey_025uM= W_coef[0]+W_coef[1]*x W_coef={29.918,1.1568} V_chisq= 1.01214;V_npnts= 4;V_numNaNs= 0;V_numINFs= 0; V_startRow= 0;V_endRow= 3;V_q= 1;V_Rab= 0.985527;V_Pr= 0.947397; V_r2= 0.89756; W_sigma={2.1,0.276} Coefficient values ± one standard deviation a =29.918 ± 2.1 b =1.1568 ± 0.276 P0 1ٻ33fit_rey_100uM @???Uk"Cݟ2@aF"6@fit_rey_100uM= W_coef[0]+W_coef[1]*x W_coef={28.076,1.0322} V_chisq= 0.749727;V_npnts= 4;V_numNaNs= 0;V_numINFs= 0; V_startRow= 0;V_endRow= 3;V_q= 1;V_Rab= 0.985527;V_Pr= 0.950795; V_r2= 0.904011; W_sigma={1.81,0.238} Coefficient values ± one standard deviation a =28.076 ± 1.81 b =1.0322 ± 0.238 h1ٻ˧KoKEhalf_pH2p5????S㥿yѩ+ o_yd*G?dp?h1ٻKڥKpH2p5_ligand_conc????h㈵>h㈵>-C6>-C6 ?-C6?Ih1ٻêK٪Kln_pH2p5_ligand_conc????MZi([*'Sz1%#Uk"Qh1ٻKKrey_pH2p5?????g=8@7@L26@ؤ&6@O}5@S`H1ٻPL8LEhalf_025uM_ver2????֋hW?xz,C? 7è?kHcCL`B1ٻwLDLEhalf_050uM_ver2????Hh˹?S"^F?J4?&N`1ٻLߝLEhalf_100uM_ver2????M֨?R񘁲?Ĭ?dp?` `1ٻ L Lrey_025uM_02????Q 2@q3@%d-p4@x7@`1ٻ3L3Lrey_050uM_02????ae2@%3@=4@ 6@!`1ٻSLSLrey_100uM_02????ބN1@"<3@r]W5@O}5@tP301ٻ@L@Lfit_rey_025uM_02 @???Uk"~bxp2@jBœ6@fit_rey_025uM_02= W_coef[0]+W_coef[1]*x W_coef={29.567,1.2081} V_chisq= 0.549603;V_npnts= 4;V_numNaNs= 0;V_numINFs= 0; V_startRow= 0;V_endRow= 3;V_q= 1;V_Rab= 0.985527;V_Pr= 0.972746; V_r2= 0.946234; W_sigma={1.55,0.204} Coefficient values ± one standard deviation a =29.567 ± 1.55 b =1.2081 ± 0.204 P3&1ٻ8L8Lfit_rey_050uM_02 @???Uk"ӷ2@Ҽ{j6@fit_rey_050uM_02= W_coef[0]+W_coef[1]*x W_coef={29.585,1.1992} V_chisq= 0.242731;V_npnts= 4;V_numNaNs= 0;V_numINFs= 0; V_startRow= 0;V_endRow= 3;V_q= 1;V_Rab= 0.985527;V_Pr= 0.987505; V_r2= 0.975166; W_sigma={1.03,0.135} Coefficient values ± one standard deviation a =29.585 ± 1.03 b =1.1992 ± 0.135 P21ٻ0L0Lfit_rey_100uM_02 @???Uk"0H5"'2@5@fit_rey_100uM_02= W_coef[0]+W_coef[1]*x W_coef={28.188,1.0896} V_chisq= 0.674614;V_npnts= 4;V_numNaNs= 0;V_numINFs= 0; V_startRow= 0;V_endRow= 3;V_q= 1;V_Rab= 0.985527;V_Pr= 0.959703; V_r2= 0.92103; W_sigma={1.71,0.226} Coefficient values ± one standard deviation a =28.188 ± 1.71 b =1.0896 ± 0.226  2019_1029_pH3p5_tjs!y@ܻAȢ?V_Flag@S_waveNames wave0;wave1;S_pathamesS_fileName Clipboards61ٻPE_pH3p5_blkP????aodʿq $ ɿ`5!ɿ0BxȿcCǿH.ǿc&ƿnnLOXſo1=ſxZĿ*<ÿq]Pÿ%:¿';¿!<8bLq@H.1澿.񝘽[tYLzk`|?5^pqtb(ൿgJA4FJvl 'iW!'ծ[Ɏ@pe1H /r]T[Cfڞv4sխ .VPst~z?+~7u?L~ބ?Zd;?IQ,?6?Y?"?IQ,?4ؙB?.4ש?"~j?:3P?$bJ$˰?n? 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DefaultFont "Arial" MoveWindow/P 939,218.75,1263,497.75 Graph2() Graph0_3() Graph10() Graph11() Graph12() Graph14() Graph17() Graph4() Table0() Table0_1() Table0_2() Table0_3() Graph6() Graph18() Table1() Graph20() Graph21() Table2() Layout0() Graph3() Graph0() Graph0_2() Graph7() Graph9() Graph8() Graph19() Graph1_1() Graph23() Graph22() Graph0_1() Graph1() Graph5() Graph16() Graph13() MoveWindow/C 15,472.25,975,669.5 Graph24() Graph15() KillStrings/Z root:gWMSetNextTextFilesTextEncoding Window Graph15() : Graph PauseUpdate; Silent 1 // building window... Display /W=(462,244.25,765.75,471.5) :'2019_1029_pH3p5_tjs':i_pH3p5_050uM_HHQ vs :'2019_1029_pH3p5_tjs':E_pH3p5_050uM_HHQ AppendToGraph :'2019_0930_pH_3p0_tjs':i_pH3_050uM_HHQ vs :'2019_0930_pH_3p0_tjs':E_pH3_050uM_HHQ AppendToGraph :'2019_0930_pH_2p5_tjs':i_pH2p5_050umHHQ vs :'2019_0930_pH_2p5_tjs':E_pH2p5_050umHHQ AppendToGraph i_pH4_050uM_HHQ vs E_pH4_050uM_HHQ AppendToGraph i_pH4_blk vs E_pH4_blk ModifyGraph gFont="Times New Roman",gfSize=15 ModifyGraph marker(i_pH3_050uM_HHQ)=19 ModifyGraph lSize(i_pH3_050uM_HHQ)=2 ModifyGraph lStyle(i_pH3_050uM_HHQ)=2,lStyle(i_pH4_blk)=3 ModifyGraph rgb(i_pH3p5_050uM_HHQ)=(4369,4369,4369),rgb(i_pH3_050uM_HHQ)=(3,52428,1) ModifyGraph rgb(i_pH2p5_050umHHQ)=(0,0,65535),rgb(i_pH4_blk)=(0,0,0) ModifyGraph msize(i_pH3_050uM_HHQ)=2 ModifyGraph tick=2 ModifyGraph mirror=1 ModifyGraph lblMargin(left)=16,lblMargin(bottom)=6 ModifyGraph axOffset(bottom)=0.318182 ModifyGraph lblLatPos(left)=-3 ModifyGraph btLen=7 ModifyGraph manTick(left)={0,0.1,-9,1},manMinor(left)={1,50} ModifyGraph manTick(bottom)={0,0.2,0,1},manMinor(bottom)={1,50} Label left "\\F'Times New Roman'\\f02I\\f00 \\u#2/nA" Label bottom "\\u#2" SetAxis left -9.2825767e-11,6e-10 SetAxis bottom -0.13156015,0.55 Cursor/P A i_pH4_050uM_HHQ 198 ShowInfo TextBox/C/N=text0/F=0/M/B=1/A=MC/X=19.85/Y=42.86 "a" TextBox/C/N=text0_1/F=0/M/B=1/A=MC/X=42.65/Y=12.86 "d" TextBox/C/N=text0_2/F=0/M/B=1/A=MC/X=36.03/Y=47.14 "b" TextBox/C/N=text0_3/F=0/M/B=1/A=MC/X=41.18/Y=36.19 "c" TextBox/C/N=text1/F=0/M/B=1/A=MC/X=35.66/Y=-14.76 "blank" ShowTools/A SetDrawLayer UserFront DrawPICT 0.363970588235294,1.12626728110599,0.347107,0.358974,PICT_0 SetDrawEnv arrow= 1 DrawLine 0.102941176470588,0.594777265745008,0.334558823529412,0.507219662058372 SetDrawEnv arrow= 1 DrawLine 0.805147058823529,0.91321044546851,0.503676470588235,0.917818740399386 EndMacro Window Graph24() : Graph PauseUpdate; Silent 1 // building window... Display /W=(517.5,133.25,732.75,424.25)/L=rey25uM rey_025uM_02 vs ln_cH AppendToGraph/L=rey50uM rey_050uM_02 vs ln_cH AppendToGraph rey_100uM_02 vs ln_cH AppendToGraph fit_rey_100uM_02 AppendToGraph/L=rey50uM fit_rey_050uM_02 AppendToGraph/L=rey25uM fit_rey_025uM_02 ModifyGraph gFont="Times New Roman",gfSize=15 ModifyGraph mode(rey_025uM_02)=3,mode(rey_050uM_02)=3,mode(rey_100uM_02)=3 ModifyGraph marker(rey_025uM_02)=19,marker(rey_050uM_02)=16,marker(rey_100uM_02)=17 ModifyGraph rgb(rey_025uM_02)=(0,0,0),rgb(rey_050uM_02)=(0,0,0),rgb(rey_100uM_02)=(0,0,0) ModifyGraph msize(rey_100uM_02)=4 ModifyGraph tick=2 ModifyGraph mirror=1 ModifyGraph fSize=14 ModifyGraph lblMargin(bottom)=12 ModifyGraph lblPos(left)=52 ModifyGraph lblLatPos(bottom)=1 ModifyGraph freePos(rey25uM)={0,kwFraction} ModifyGraph freePos(rey50uM)={0,kwFraction} ModifyGraph axisEnab(rey25uM)={0.69,1} ModifyGraph axisEnab(rey50uM)={0.35,0.63} ModifyGraph axisEnab(left)={0,0.3} ModifyGraph manTick(rey25uM)={0,2,0,0},manMinor(rey25uM)={1,50} ModifyGraph manTick(bottom)={0,1,0,0},manMinor(bottom)={1,50} ModifyGraph manTick(rey50uM)={0,2,0,0},manMinor(rey50uM)={1,50} ModifyGraph manTick(left)={0,2,0,0},manMinor(left)={1,50} Label bottom "ln[c\\BH\\S+\\M]" SetAxis/A/N=2 rey25uM SetAxis/A/N=2 bottom SetAxis/A/N=2 rey50uM SetAxis/A/N=2 left TextBox/C/N=text0/F=0/A=MC/X=-40.00/Y=43.51 "\\Z18\\f01A" TextBox/C/N=text0_1/F=0/A=MC/X=-40.00/Y=9.09 "\\Z18\\f01B" TextBox/C/N=text0_2/F=0/A=MC/X=-40.00/Y=-23.70 "\\Z18\\f01C" ShowTools/A SetDrawLayer UserFront DrawPICT -0.440251572327044,0.195876288659794,0.170124,0.14837,PICT_6 EndMacro Window Graph13() : Graph PauseUpdate; Silent 1 // building window... String fldrSav0= GetDataFolder(1) SetDataFolder root:'2019_0930_pH_2p5_tjs': Display /W=(986.25,389.75,1380.75,598.25) i_pH2p5_050umHHQ vs E_pH2p5_050umHHQ SetDataFolder fldrSav0 ModifyGraph gFont="Times New Roman",gfSize=15 ModifyGraph tick=2 ModifyGraph mirror=1 ModifyGraph lblMargin(left)=16 ModifyGraph lblLatPos(left)=-3 ModifyGraph btLen=7 ModifyGraph manTick(left)={0,200,-12,0},manMinor(left)={1,50} ModifyGraph manTick(bottom)={0,0.2,0,1},manMinor(bottom)={1,50} Label left "\\F'Times New Roman'\\f02I\\f00 1 × \\u /A" Label bottom "\\F'Times New Roman'E /V" Cursor/P A i_pH2p5_050umHHQ 50 ShowInfo MoveWindow 0, 0, 0, 0 // Minimize the window. EndMacro Window Graph16() : Graph PauseUpdate; Silent 1 // building window... Display /W=(966.75,355.25,1361.25,563.75) i_pH4_025uM_HHQ vs E_pH4_025uM_HHQ AppendToGraph :'2019_1029_pH3p5_tjs':i_pH3p5_025uM_HHQ vs :'2019_1029_pH3p5_tjs':E_pH3p5_025uM_HHQ AppendToGraph :'2019_0930_pH_3p0_tjs':i_pH3_025uM_HHQ vs :'2019_0930_pH_3p0_tjs':E_pH3_025uM_HHQ AppendToGraph :'2019_0930_pH_2p5_tjs':i_pH2p5_025umHHQ vs :'2019_0930_pH_2p5_tjs':E_pH2p5_025umHHQ ModifyGraph gFont="Times New Roman",gfSize=15 ModifyGraph lSize(i_pH3_025uM_HHQ)=2 ModifyGraph lStyle(i_pH3_025uM_HHQ)=2 ModifyGraph rgb(i_pH3p5_025uM_HHQ)=(4369,4369,4369),rgb(i_pH3_025uM_HHQ)=(3,52428,1) ModifyGraph rgb(i_pH2p5_025umHHQ)=(0,0,65535) ModifyGraph tick=2 ModifyGraph mirror=1 ModifyGraph lblMargin(left)=16 ModifyGraph lblLatPos(left)=-3 ModifyGraph btLen=7 ModifyGraph manTick(left)={0,200,-12,0},manMinor(left)={1,50} ModifyGraph manTick(bottom)={0,0.2,0,1},manMinor(bottom)={1,50} Label left "\\F'Times New Roman'\\f02I\\f00 1 × \\u /A" Label bottom "\\F'Times New Roman'E /V" Cursor/P A i_pH2p5_025umHHQ 47 ShowInfo MoveWindow 0, 0, 0, 0 // Minimize the window. EndMacro Window Graph5() : Graph PauseUpdate; Silent 1 // building window... Display /W=(970.5,374.75,1365,583.25) i_pH4_100uM_HHQ vs E_pH4_100uM_HHQ AppendToGraph :'2019_0930_pH_2p5_tjs':i_pH2p5_100umHHQ vs :'2019_0930_pH_2p5_tjs':E_pH2p5_100umHHQ AppendToGraph :'2019_0930_pH_3p0_tjs':i_pH3_100uM_HHQ vs :'2019_0930_pH_3p0_tjs':E_pH3_100uM_HHQ_2 AppendToGraph :'2019_1029_pH3p5_tjs':i_pH3p5_100uM_HHQ vs :'2019_1029_pH3p5_tjs':E_pH3p5_100uM_HHQ ModifyGraph gFont="Times New Roman",gfSize=15 ModifyGraph rgb(i_pH2p5_100umHHQ)=(1,4,52428),rgb(i_pH3_100uM_HHQ)=(1,52428,26586) ModifyGraph rgb(i_pH3p5_100uM_HHQ)=(0,0,0) ModifyGraph tick=2 ModifyGraph mirror=1 ModifyGraph lblMargin(left)=16 ModifyGraph lblLatPos(left)=-3 ModifyGraph btLen=7 ModifyGraph manTick(left)={0,200,-12,0},manMinor(left)={1,50} ModifyGraph manTick(bottom)={0,0.2,0,1},manMinor(bottom)={1,50} Label left "\\F'Times New Roman'\\f02I\\f00 1 × \\u /A" Label bottom "\\F'Times New Roman'E /V" Cursor/P A i_pH2p5_100umHHQ 46 ShowInfo MoveWindow 0, 0, 0, 0 // Minimize the window. EndMacro Window Graph1() : Graph PauseUpdate; Silent 1 // building window... String fldrSav0= GetDataFolder(1) SetDataFolder root:'2019_0930_pH_3p0_tjs': Display /W=(968.25,395.75,1362.75,604.25) i_pH3_100uM_HHQ vs E_pH3_100uM_HHQ_2 AppendToGraph i_pH3_blk vs E_pH3_blk_2 AppendToGraph i_pH3_005uM_HHQ vs E_pH3_005uM_HHQ AppendToGraph i_pH3_010uM_HHQ vs E_pH3_010uM_HHQ AppendToGraph i_pH3_025uM_HHQ vs E_pH3_025uM_HHQ AppendToGraph i_pH3_050uM_HHQ vs E_pH3_050uM_HHQ SetDataFolder fldrSav0 ModifyGraph gFont="Times New Roman",gfSize=15 ModifyGraph tick=2 ModifyGraph mirror=1 ModifyGraph lblMargin(left)=16 ModifyGraph lblLatPos(left)=-3 ModifyGraph btLen=7 ModifyGraph manTick(left)={0,200,-12,0},manMinor(left)={1,50} ModifyGraph manTick(bottom)={0,0.2,0,1},manMinor(bottom)={1,50} Label left "\\F'Times New Roman'\\f02I\\f00 1 × \\u /A" Label bottom "\\F'Times New Roman'E /V" MoveWindow 0, 0, 0, 0 // Minimize the window. EndMacro Window Graph0_1() : Graph PauseUpdate; Silent 1 // building window... String fldrSav0= GetDataFolder(1) SetDataFolder root:'2019_1029_pH3p5_tjs': Display /W=(969,416.75,1363.5,625.25) i_pH3p5_blk vs E_pH3p5_blk AppendToGraph i_pH3p5_100uM_HHQ vs E_pH3p5_100uM_HHQ AppendToGraph i_pH3p5_050uM_HHQ vs E_pH3p5_050uM_HHQ AppendToGraph i_pH3p5_025uM_HHQ vs E_pH3p5_025uM_HHQ AppendToGraph i_pH3p5_010uM_HHQ vs E_pH3p5_010uM_HHQ AppendToGraph i_pH3p5_005uM_HHQ vs E_pH3p5_005uM_HHQ SetDataFolder fldrSav0 ModifyGraph gFont="Times New Roman",gfSize=15 ModifyGraph tick=2 ModifyGraph mirror=1 ModifyGraph lblMargin(left)=16 ModifyGraph lblLatPos(left)=-3 ModifyGraph btLen=7 ModifyGraph manTick(left)={0,200,-12,0},manMinor(left)={1,50} ModifyGraph manTick(bottom)={0,0.2,0,1},manMinor(bottom)={1,50} Label left "\\F'Times New Roman'\\f02I\\f00 1 × \\u /A" Label bottom "\\F'Times New Roman'E /V" MoveWindow 0, 0, 0, 0 // Minimize the window. EndMacro Window Graph22() : Graph PauseUpdate; Silent 1 // building window... Display /W=(398.25,60.5,792.75,269) i_pH4_100uM_HHQ vs E_pH4_100uM_HHQ AppendToGraph :'2019_1029_pH3p5_tjs':i_pH3p5_100uM_HHQ vs :'2019_1029_pH3p5_tjs':E_pH3p5_100uM_HHQ AppendToGraph :'2019_0930_pH_3p0_tjs':i_pH3_100uM_HHQ vs :'2019_0930_pH_3p0_tjs':E_pH3_100uM_HHQ_2 AppendToGraph :'2019_0930_pH_2p5_tjs':i_pH2p5_100umHHQ vs :'2019_0930_pH_2p5_tjs':E_pH2p5_100umHHQ ModifyGraph rgb(i_pH4_100uM_HHQ)=(0,0,65535),rgb(i_pH3p5_100uM_HHQ)=(26205,52428,1) ModifyGraph rgb(i_pH3_100uM_HHQ)=(0,0,0) SetAxis left -8.57849573459716e-11,4.86441440758294e-10 SetAxis bottom -0.186339858823529,0.3657212 Cursor/P A i_pH2p5_100umHHQ 47 ShowInfo EndMacro Window Graph23() : Graph PauseUpdate; Silent 1 // building window... Display /W=(459.75,338.75,854.25,547.25) rey_025uM_02,rey_050uM_02,rey_100uM_02 vs ln_cH AppendToGraph fit_rey_025uM_02,fit_rey_050uM_02,fit_rey_100uM_02 ModifyGraph mode(rey_025uM_02)=3,mode(rey_050uM_02)=3,mode(rey_100uM_02)=3 ModifyGraph marker(rey_025uM_02)=19,marker(rey_050uM_02)=16,marker(rey_100uM_02)=17 ModifyGraph rgb(rey_025uM_02)=(0,0,0),rgb(rey_050uM_02)=(0,0,0),rgb(rey_100uM_02)=(0,0,0) EndMacro Window Graph1_1() : Graph PauseUpdate; Silent 1 // building window... String fldrSav0= GetDataFolder(1) SetDataFolder root:'2019_0930_pH_2p5_tjs': Display /W=(902.25,231.5,1296.75,440) i_pH2p5_005umHHQ vs E_pH2p5_005umHHQ AppendToGraph i_pH2p5_010umHHQ vs E_pH2p5_010umHHQ AppendToGraph i_pH2p5_025umHHQ vs E_pH2p5_025umHHQ AppendToGraph i_pH2p5_050umHHQ vs E_pH2p5_050umHHQ AppendToGraph i_pH2p5_100umHHQ vs E_pH2p5_100umHHQ SetDataFolder fldrSav0 ModifyGraph gFont="Times New Roman",gfSize=15 ModifyGraph tick=2 ModifyGraph mirror=1 ModifyGraph lblMargin(left)=8 ModifyGraph btLen=7 ModifyGraph manTick(left)={0,0.2,-9,1},manMinor(left)={1,50} ModifyGraph manTick(bottom)={0,0.2,0,1},manMinor(bottom)={1,50} Label left "\\F'Times New Roman'\\f02I\\f00 1 × \\u /A" Label bottom "\\F'Times New Roman'E /V" Cursor/P A i_pH2p5_100umHHQ 47 ShowInfo MoveWindow 0, 0, 0, 0 // Minimize the window. EndMacro Window Graph19() : Graph PauseUpdate; Silent 1 // building window... Display /W=(826.5,227.75,1221,436.25) rey_pH2p5 vs ln_pH2p5_ligand_conc MoveWindow 0, 0, 0, 0 // Minimize the window. EndMacro Window Graph8() : Graph PauseUpdate; Silent 1 // building window... Display /W=(558.75,248.75,953.25,457.25) i_pH4_100uM_HHQ vs E_pH4_100uM_HHQ ModifyGraph gFont="Times New Roman",gfSize=15 ModifyGraph tick=2 ModifyGraph mirror=1 ModifyGraph lblMargin(left)=16 ModifyGraph lblLatPos(left)=-3 ModifyGraph btLen=7 ModifyGraph manTick(left)={0,200,-12,0},manMinor(left)={1,50} ModifyGraph manTick(bottom)={0,0.2,0,1},manMinor(bottom)={1,50} Label left "\\F'Times New Roman'\\f02I\\f00 1 × \\u /A" Label bottom "\\F'Times New Roman'E /V" Cursor/P A i_pH4_100uM_HHQ 63 ShowInfo MoveWindow 0, 0, 0, 0 // Minimize the window. EndMacro Window Graph9() : Graph PauseUpdate; Silent 1 // building window... Display /W=(558,253.25,952.5,461.75) rey_100uM vs ln_cH ModifyGraph gFont="Times New Roman",gfSize=15 ModifyGraph mode=3 ModifyGraph marker=8 ModifyGraph rgb=(0,0,0) ModifyGraph msize=2.5 ModifyGraph tick=2 ModifyGraph mirror=1 ModifyGraph lblMargin(left)=16 ModifyGraph lblLatPos(left)=-3 ModifyGraph btLen=7 ModifyGraph manTick(left)={0,0.5,0,1},manMinor(left)={0,50} ModifyGraph manTick(bottom)={0,0.5,0,1},manMinor(bottom)={0,50} Label left "\\F'Times New Roman'\\f02I\\f00 1 × \\u /A" Label bottom "\\F'Times New Roman'E /V" MoveWindow 0, 0, 0, 0 // Minimize the window. EndMacro Window Graph7() : Graph PauseUpdate; Silent 1 // building window... String fldrSav0= GetDataFolder(1) SetDataFolder root:'2019_1029_pH3p5_tjs': Display /W=(483,276.5,877.5,485) i_pH3p5_100uM_HHQ vs E_pH3p5_100uM_HHQ SetDataFolder fldrSav0 ModifyGraph gFont="Times New Roman",gfSize=15 ModifyGraph tick=2 ModifyGraph mirror=1 ModifyGraph lblMargin(left)=16 ModifyGraph lblLatPos(left)=-3 ModifyGraph btLen=7 ModifyGraph manTick(left)={0,200,-12,0},manMinor(left)={1,50} ModifyGraph manTick(bottom)={0,0.2,0,1},manMinor(bottom)={1,50} Label left "\\F'Times New Roman'\\f02I\\f00 1 × \\u /A" Label bottom "\\F'Times New Roman'E /V" Cursor/P A i_pH3p5_100uM_HHQ 56 ShowInfo MoveWindow 0, 0, 0, 0 // Minimize the window. EndMacro Window Graph0_2() : Graph PauseUpdate; Silent 1 // building window... String fldrSav0= GetDataFolder(1) SetDataFolder root:'2019_0930_pH_3p0_tjs': Display /W=(375,66.5,769.5,275) i_pH3_blk vs E_pH3_blk AppendToGraph i_pH3_100uM_HHQ vs E_pH3_100uM_HHQ SetDataFolder fldrSav0 ModifyGraph gFont="Times New Roman",gfSize=15 ModifyGraph tick=2 ModifyGraph mirror=1 ModifyGraph lblMargin(left)=16 ModifyGraph lblLatPos(left)=-3 ModifyGraph btLen=7 ModifyGraph manTick(left)={0,200,-12,0},manMinor(left)={1,50} ModifyGraph manTick(bottom)={0,0.2,0,1},manMinor(bottom)={1,50} Label left "\\F'Times New Roman'\\f02I\\f00 1 × \\u /A" Label bottom "\\F'Times New Roman'E /V" ShowInfo MoveWindow 0, 0, 0, 0 // Minimize the window. EndMacro Window Graph0() : Graph PauseUpdate; Silent 1 // building window... Display /W=(920.25,59.75,1314.75,268.25) i_pH4_blk vs E_pH4_blk AppendToGraph i_pH4_010uM_HHQ vs E_pH4_010uM_HHQ AppendToGraph i_pH4_025uM_HHQ vs E_pH4_025uM_HHQ AppendToGraph i_pH4_050uM_HHQ vs E_pH4_050uM_HHQ AppendToGraph i_pH4_100uM_HHQ vs E_pH4_100uM_HHQ AppendToGraph i_pH4_500uM_HHQ vs E_pH4_500uM_HHQ AppendToGraph i_pH4_250uM_HHQ vs E_pH4_250uM_HHQ ModifyGraph gFont="Times New Roman",gfSize=15 ModifyGraph rgb(i_pH4_025uM_HHQ)=(0,0,65535) ModifyGraph tick=2 ModifyGraph mirror=1 ModifyGraph lblMargin(left)=16 ModifyGraph lblLatPos(left)=-3 ModifyGraph btLen=7 ModifyGraph manTick(left)={0,200,-12,0},manMinor(left)={0,50} ModifyGraph manTick(bottom)={0,0.2,0,1},manMinor(bottom)={1,50} Label left "\\F'Times New Roman'\\f02I\\f00 1 × \\u /A" Label bottom "\\F'Times New Roman'E /V" Cursor/P A i_pH4_025uM_HHQ 391 ShowInfo MoveWindow 0, 0, 0, 0 // Minimize the window. EndMacro Window Graph3() : Graph PauseUpdate; Silent 1 // building window... String fldrSav0= GetDataFolder(1) SetDataFolder root:'2019_0930_pH_2p5_tjs': Display /W=(788.25,312.5,1182.75,521) i_pH2p5_025umHHQ vs E_pH2p5_025umHHQ SetDataFolder fldrSav0 ModifyGraph gFont="Times New Roman",gfSize=15 ModifyGraph tick=2 ModifyGraph mirror=1 ModifyGraph lblMargin(left)=16 ModifyGraph lblLatPos(left)=-3 ModifyGraph btLen=7 ModifyGraph manTick(left)={0,200,-12,0},manMinor(left)={1,50} ModifyGraph manTick(bottom)={0,0.2,0,1},manMinor(bottom)={1,50} Label left "\\F'Times New Roman'\\f02I\\f00 1 × \\u /A" Label bottom "\\F'Times New Roman'E /V" MoveWindow 0, 0, 0, 0 // Minimize the window. EndMacro Window Layout0() : Layout PauseUpdate; Silent 1 // building window... NewLayout/W=(250.5,179.75,756.75,633.5) if (IgorVersion() >= 7.00) LayoutPageAction size=(612,792),margins=(18,18,18,18) endif AppendLayoutObject/F=0/T=0/R=(48,48,468,453) Graph Graph24 SetDrawLayer UserFront DrawPICT 39,135,0.275,0.216123,PICT_5 MoveWindow 0, 0, 0, 0 // Minimize the window. EndMacro Window Table2() : Table PauseUpdate; Silent 1 // building window... Edit/W=(71.25,209.75,576,416.75) pH,ln_cH,Ehalf_025uM_ver2,Ehalf_050uM_ver2,Ehalf_100uM_ver2 AppendToTable rey_025uM_02,rey_050uM_02,rey_100uM_02 ModifyTable format(Point)=1 EndMacro Window Graph21() : Graph PauseUpdate; Silent 1 // building window... Display /W=(661.5,76.25,1056,284.75) i_pH4_050uM_HHQ vs E_pH4_050uM_HHQ AppendToGraph :'2019_1029_pH3p5_tjs':i_pH3p5_050uM_HHQ vs :'2019_1029_pH3p5_tjs':E_pH3p5_050uM_HHQ AppendToGraph :'2019_0930_pH_3p0_tjs':i_pH3_050uM_HHQ vs :'2019_0930_pH_3p0_tjs':E_pH3_050uM_HHQ AppendToGraph i_pH4_050uM_HHQ[0,332] vs :'2019_0930_pH_2p5_tjs':E_pH2p5_050umHHQ ModifyGraph rgb(i_pH4_050uM_HHQ)=(0,0,65535),rgb(i_pH3p5_050uM_HHQ)=(26205,52428,1) ModifyGraph rgb(i_pH3_050uM_HHQ)=(0,0,0) SetAxis left -4.61477488151658e-11,2.60970218009479e-10 SetAxis bottom -0.246639717647059,0.355952705882353 Cursor/P A i_pH4_050uM_HHQ#1 50 ShowInfo EndMacro Window Graph20() : Graph PauseUpdate; Silent 1 // building window... Display /W=(658.5,57.5,1053,266) i_pH4_025uM_HHQ vs E_pH4_025uM_HHQ AppendToGraph :'2019_1029_pH3p5_tjs':i_pH3p5_025uM_HHQ vs :'2019_1029_pH3p5_tjs':E_pH3p5_025uM_HHQ AppendToGraph :'2019_0930_pH_3p0_tjs':i_pH3_025uM_HHQ vs :'2019_0930_pH_3p0_tjs':E_pH3_025uM_HHQ AppendToGraph :'2019_0930_pH_2p5_tjs':i_pH2p5_025umHHQ vs :'2019_0930_pH_2p5_tjs':E_pH2p5_025umHHQ ModifyGraph rgb(i_pH4_025uM_HHQ)=(0,0,65535),rgb(i_pH3p5_025uM_HHQ)=(26205,52428,1) ModifyGraph rgb(i_pH3_025uM_HHQ)=(0,0,0) SetAxis left -6.3175625592417e-11,2.15630440758294e-10 SetAxis bottom -0.134908352941176,0.290314752941176 Cursor/P A i_pH2p5_025umHHQ 51 ShowInfo EndMacro Window Table1() : Table PauseUpdate; Silent 1 // building window... Edit/W=(90,128,676.5,335) pH,conc_H,Ehalf_100uM,ln_cH,rey_100uM,Ehalf_050uM,rey_050uM AppendToTable Ehalf_HHQ_050uM,Ehalf_025uM,rey_025uM,Ehalf_pH2p5,pH2p5_ligand_conc AppendToTable ln_pH2p5_ligand_conc,rey_pH2p5 ModifyTable format(Point)=1 EndMacro Window Graph18() : Graph PauseUpdate; Silent 1 // building window... Display /W=(181.5,75.5,576,284) rey_100uM vs ln_cH AppendToGraph fit_rey_100uM ModifyGraph gFont="Times New Roman",gfSize=15 ModifyGraph mode(rey_100uM)=3 ModifyGraph marker(rey_100uM)=19 ModifyGraph rgb(rey_100uM)=(0,0,0) ModifyGraph msize(rey_100uM)=2 ModifyGraph tick=2 ModifyGraph mirror=1 ModifyGraph lblMargin(left)=16 ModifyGraph lblLatPos(left)=-3 ModifyGraph btLen=7 ModifyGraph manTick(left)={0,0.5,0,1},manMinor(left)={0,50} ModifyGraph manTick(bottom)={0,0.5,0,1},manMinor(bottom)={0,50} Label left "\\F'Times New Roman'\\f02I\\f00 1 × \\u /A" Label bottom "\\F'Times New Roman'E /V" EndMacro Window Graph6() : Graph PauseUpdate; Silent 1 // building window... String fldrSav0= GetDataFolder(1) SetDataFolder root:'2019_0930_pH_3p0_tjs': Display /W=(672.75,42.5,1067.25,251) i_pH3_100uM_HHQ vs E_pH3_100uM_HHQ_2 SetDataFolder fldrSav0 ModifyGraph gFont="Times New Roman",gfSize=15 ModifyGraph tick=2 ModifyGraph mirror=1 ModifyGraph lblMargin(left)=16 ModifyGraph lblLatPos(left)=-3 ModifyGraph btLen=7 ModifyGraph manTick(left)={0,200,-12,0},manMinor(left)={1,50} ModifyGraph manTick(bottom)={0,0.2,0,1},manMinor(bottom)={1,50} Label left "\\F'Times New Roman'\\f02I\\f00 1 × \\u /A" Label bottom "\\F'Times New Roman'E /V" Cursor/P A i_pH3_100uM_HHQ 60 ShowInfo EndMacro Window Table0_3() : Table PauseUpdate; Silent 1 // building window... String fldrSav0= GetDataFolder(1) SetDataFolder root:'2019_0930_pH_2p5_tjs': Edit/W=(90.75,62,656.25,520.25) i_pH2p5_blk,E_pH2p5_blk,i_pH2p5_005umHHQ,E_pH2p5_005umHHQ AppendToTable i_pH2p5_010umHHQ,E_pH2p5_010umHHQ,i_pH2p5_025umHHQ,E_pH2p5_025umHHQ AppendToTable i_pH2p5_050umHHQ,E_pH2p5_050umHHQ,i_pH2p5_100umHHQ,E_pH2p5_100umHHQ ModifyTable format(Point)=1 SetDataFolder fldrSav0 MoveWindow 0, 0, 0, 0 // Minimize the window. EndMacro Window Table0_2() : Table PauseUpdate; Silent 1 // building window... String fldrSav0= GetDataFolder(1) SetDataFolder root:'2019_0930_pH_3p0_tjs': Edit/W=(124.5,268.25,748.5,475.25) i_pH3_blk,E_pH3_blk,i_pH3_100uM_HHQ,E_pH3_100uM_HHQ AppendToTable E_pH3_100uM_HHQ_2,E_pH3_blk_2,E_pH3_005uM_HHQ,i_pH3_005uM_HHQ,E_pH3_010uM_HHQ AppendToTable i_pH3_010uM_HHQ,E_pH3_025uM_HHQ,i_pH3_025uM_HHQ,E_pH3_050uM_HHQ,i_pH3_050uM_HHQ AppendToTable conc_HHQ,j_uAcm2 ModifyTable format(Point)=1 SetDataFolder fldrSav0 MoveWindow 0, 0, 0, 0 // Minimize the window. EndMacro Window Table0_1() : Table PauseUpdate; Silent 1 // building window... String fldrSav0= GetDataFolder(1) SetDataFolder root:'2019_1029_pH3p5_tjs': Edit/W=(93.75,182.75,959.25,389.75) E_pH3p5_blk,i_pH3p5_blk,E_pH3p5_100uM_HHQ,i_pH3p5_100uM_HHQ AppendToTable E_pH3p5_050uM_HHQ,i_pH3p5_050uM_HHQ,E_pH3p5_025uM_HHQ,i_pH3p5_025uM_HHQ AppendToTable E_pH3p5_010uM_HHQ,i_pH3p5_010uM_HHQ,E_pH3p5_005uM_HHQ,i_pH3p5_005uM_HHQ ModifyTable format(Point)=1 SetDataFolder fldrSav0 MoveWindow 0, 0, 0, 0 // Minimize the window. EndMacro Window Table0() : Table PauseUpdate; Silent 1 // building window... Edit/W=(78.75,64.25,744,549.5) E_pH4_blk,i_pH4_blk,E_pH4_010uM_HHQ,i_pH4_010uM_HHQ AppendToTable E_pH4_025uM_HHQ,i_pH4_025uM_HHQ,E_pH4_050uM_HHQ,i_pH4_050uM_HHQ,E_pH4_100uM_HHQ AppendToTable i_pH4_100uM_HHQ,E_pH4_500uM_HHQ,i_pH4_500uM_HHQ,E_pH4_250uM_HHQ,i_pH4_250uM_HHQ AppendToTable conc_HHQ,j_uAcm2 ModifyTable format(Point)=1 MoveWindow 0, 0, 0, 0 // Minimize the window. EndMacro Window Graph4() : Graph PauseUpdate; Silent 1 // building window... String fldrSav0= GetDataFolder(1) SetDataFolder root:'2019_0930_pH_2p5_tjs': Display /W=(503.25,257,897.75,465.5) i_pH2p5_100umHHQ vs E_pH2p5_100umHHQ SetDataFolder fldrSav0 ModifyGraph gFont="Times New Roman",gfSize=15 ModifyGraph tick=2 ModifyGraph mirror=1 ModifyGraph lblMargin(left)=16 ModifyGraph lblLatPos(left)=-3 ModifyGraph btLen=7 ModifyGraph manTick(left)={0,200,-12,0},manMinor(left)={1,50} ModifyGraph manTick(bottom)={0,0.2,0,1},manMinor(bottom)={1,50} Label left "\\F'Times New Roman'\\f02I\\f00 1 × \\u /A" Label bottom "\\F'Times New Roman'E /V" MoveWindow 0, 0, 0, 0 // Minimize the window. EndMacro Window Graph17() : Graph PauseUpdate; Silent 1 // building window... Display /W=(144.75,149,539.25,357.5) rey_025uM vs ln_cH AppendToGraph fit_rey_025uM ModifyGraph gFont="Times New Roman",gfSize=15 ModifyGraph mode(rey_025uM)=3 ModifyGraph marker(rey_025uM)=19 ModifyGraph rgb(rey_025uM)=(0,0,0) ModifyGraph msize(rey_025uM)=2 ModifyGraph tick=2 ModifyGraph mirror=1 ModifyGraph lblMargin(left)=16 ModifyGraph lblLatPos(left)=-3 ModifyGraph btLen=7 ModifyGraph manTick(left)={0,1,0,0},manMinor(left)={0,50} ModifyGraph manTick(bottom)={0,0.5,0,1},manMinor(bottom)={1,50} Label left "\\F'Times New Roman'\\f02I\\f00 1 × \\u /A" Label bottom "\\F'Times New Roman'E /V" EndMacro Window Graph14() : Graph PauseUpdate; Silent 1 // building window... Display /W=(130.5,56.75,525,265.25) rey_050uM vs ln_cH AppendToGraph fit_rey_050uM ModifyGraph gFont="Times New Roman",gfSize=15 ModifyGraph mode(rey_050uM)=3 ModifyGraph marker(rey_050uM)=8 ModifyGraph rgb(rey_050uM)=(0,0,0) ModifyGraph msize(rey_050uM)=2.5 ModifyGraph tick=2 ModifyGraph mirror=1 ModifyGraph lblMargin(left)=16 ModifyGraph lblLatPos(left)=-3 ModifyGraph btLen=7 ModifyGraph manTick(left)={0,0.5,0,1},manMinor(left)={0,50} ModifyGraph manTick(bottom)={0,0.5,0,1},manMinor(bottom)={0,50} Label left "\\F'Times New Roman'\\f02I\\f00 1 × \\u /A" Label bottom "\\F'Times New Roman'E /V" EndMacro Window Graph12() : Graph PauseUpdate; Silent 1 // building window... String fldrSav0= GetDataFolder(1) SetDataFolder root:'2019_0930_pH_3p0_tjs': Display /W=(723.75,167.75,1118.25,376.25) i_pH3_050uM_HHQ vs E_pH3_050uM_HHQ SetDataFolder fldrSav0 ModifyGraph gFont="Times New Roman",gfSize=15 ModifyGraph tick=2 ModifyGraph mirror=1 ModifyGraph lblMargin(left)=16 ModifyGraph lblLatPos(left)=-3 ModifyGraph btLen=7 ModifyGraph manTick(left)={0,200,-12,0},manMinor(left)={1,50} ModifyGraph manTick(bottom)={0,0.2,0,1},manMinor(bottom)={1,50} Label left "\\F'Times New Roman'\\f02I\\f00 1 × \\u /A" Label bottom "\\F'Times New Roman'E /V" Cursor/P A i_pH3_050uM_HHQ 63 ShowInfo EndMacro Window Graph11() : Graph PauseUpdate; Silent 1 // building window... String fldrSav0= GetDataFolder(1) SetDataFolder root:'2019_1029_pH3p5_tjs': Display /W=(753.75,221,1148.25,429.5) i_pH3p5_050uM_HHQ vs E_pH3p5_050uM_HHQ SetDataFolder fldrSav0 ModifyGraph gFont="Times New Roman",gfSize=15 ModifyGraph tick=2 ModifyGraph mirror=1 ModifyGraph lblMargin(left)=16 ModifyGraph lblLatPos(left)=-3 ModifyGraph btLen=7 ModifyGraph manTick(left)={0,200,-12,0},manMinor(left)={1,50} ModifyGraph manTick(bottom)={0,0.2,0,1},manMinor(bottom)={1,50} Label left "\\F'Times New Roman'\\f02I\\f00 1 × \\u /A" Label bottom "\\F'Times New Roman'E /V" Cursor/P A i_pH3p5_050uM_HHQ 45 ShowInfo EndMacro Window Graph10() : Graph PauseUpdate; Silent 1 // building window... Display /W=(831,287.75,1225.5,496.25) i_pH4_050uM_HHQ vs E_pH4_050uM_HHQ ModifyGraph gFont="Times New Roman",gfSize=15 ModifyGraph tick=2 ModifyGraph mirror=1 ModifyGraph lblMargin(left)=16 ModifyGraph lblLatPos(left)=-3 ModifyGraph btLen=7 ModifyGraph manTick(left)={0,200,-12,0},manMinor(left)={1,50} ModifyGraph manTick(bottom)={0,0.2,0,1},manMinor(bottom)={1,50} Label left "\\F'Times New Roman'\\f02I\\f00 1 × \\u /A" Label bottom "\\F'Times New Roman'E /V" Cursor/P A i_pH4_050uM_HHQ 47 ShowInfo EndMacro Window Graph0_3() : Graph PauseUpdate; Silent 1 // building window... String fldrSav0= GetDataFolder(1) SetDataFolder root:'2019_0930_pH_2p5_tjs': Display /W=(739.5,284,1134,492.5) i_pH2p5_blk vs E_pH2p5_blk AppendToGraph i_pH2p5_005umHHQ vs E_pH2p5_005umHHQ SetDataFolder fldrSav0 ModifyGraph gFont="Times New Roman",gfSize=15 ModifyGraph rgb(i_pH2p5_005umHHQ)=(0,0,65535) ModifyGraph tick=2 ModifyGraph mirror=1 ModifyGraph lblMargin(left)=16 ModifyGraph lblLatPos(left)=-3 ModifyGraph manTick(left)={0,200,-12,0},manMinor(left)={0,50} Label left "\\F'Times New Roman'\\f02I\\f00 1 × \\u /A" Label bottom "\\F'Times New Roman'E /V" MoveWindow 0, 0, 0, 0 // Minimize the window. EndMacro Window Graph2() : Graph PauseUpdate; Silent 1 // building window... String fldrSav0= GetDataFolder(1) SetDataFolder root:'2019_0930_pH_2p5_tjs': Display /W=(1052.25,74,1446.75,282.5) i_pH2p5_010umHHQ vs E_pH2p5_010umHHQ AppendToGraph i_pH2p5_050umHHQ vs E_pH2p5_050umHHQ SetDataFolder fldrSav0 ModifyGraph gFont="Times New Roman",gfSize=15 ModifyGraph tick=2 ModifyGraph mirror=1 ModifyGraph lblMargin(left)=16 ModifyGraph lblLatPos(left)=-3 ModifyGraph btLen=7 ModifyGraph manTick(left)={0,200,-12,0},manMinor(left)={1,50} ModifyGraph manTick(bottom)={0,0.2,0,1},manMinor(bottom)={1,50} Label left "\\F'Times New Roman'\\f02I\\f00 1 × \\u /A" Label bottom "\\F'Times New Roman'E /V" EndMacro #pragma TextEncoding = "UTF-8" #pragma rtGlobals=3 // Use modern global access method and strict wave access. #include constant Fara = 96485.33//C mol-1 constant tem = 298.15// K constant Ry = 8.314//J mol-1 K-1 constant Eo = 0.580//V ~ transfer potential for H+ function jane01(w1) wave w1 duplicate/O w1, temp temp = -Fara/(Ry*tem)*(w1-Eo) rename temp, rey_100uM_02; end